Molecular
Diagnostic Laboratory
Nordic
Centre of Excellence in Molecular Medicine
2006 | 2005
| 2004 | 2003
|1996- 2002| By
author | Supplementary
data | NormFinder |
PhDs
Supplementary data for selected publications
| Olesen,S.H., Thykjaer,T., Orntoft,T.F. Mitotic checkpoint genes hBUB1, hBUB1B, hBUB3 and TTK in human bladder cancer, screening for mutations and loss of heterozygosity. Carcinogenesis. 22(5): 813-815. 2001. PubMed | |||
|
Primers for sequence analysis |
|||
| The shaded are PCR and sequence primers, the non-shaded are sequence primers. | |||
| Gene | Notation | Sequence | Distance in bp from translational starting point |
| hBUB1 | |||
| PCR-product 1 | |||
| PCR1S | gCg gTT CAg gTT TgC CgC TgC Cg | -39 | |
| Q1AS | CgC CCA ggC AAT gTA CAg Ag | 299 | |
| Q2S | ggA gAg CTg CAg CAT gCC AgT g | 337 | |
| Q2AS | TCT AgC TTg AgC Tgg CAA ATg g | 450 | |
| Q3S | CTg TgC ACT CAT CTT Tgg CAT CC | 659 | |
| Q3AS | CAT TTA CCC ATT gCT CAT gCT TTC | 788 | |
| PCR2AS | CAT ggA gTC TgT TAC TgT CTg | 1171 | |
| PCR-product 2 | |||
| PCR3S | gTg gAg ACA TCC CAT gAg gAT C | 910 | |
| Q5S | CAg ACT CCA TgT TTg CAg Tgg C | 1181 | |
| Q5AS | gAT CTA gAg ATT gCC ATT CAT C | 1462 | |
| Q6S | gAA gAT gCA TTT gAA gCC CAg TTT C | 1489 | |
| Q7S | CTg gCA CCC AgT CCT AAg AgC | 1768 | |
| Q6AS | ggA ATg gTg TAg ACg CAA gTT g | 1813 | |
| PCR4AS | ggA TCT TCT gCA ATg gCA gCg | 2112 | |
| PCR-product 3 | |||
| PCR5S | AgC CCA AAA CAg gCC TTg TCg | 1980 | |
| Q9S | TgT AAA CTT CCA gCC ATC AAg CCC | 2305 | |
| Q8AS | CTC CCT ggg TAg CTT CgT ACA C | 2401 | |
| Q10S | CCA CTT ATT CCA gAA Tgg CAg Tg | 2565 | |
| Q9AS | gAT TTC ACA gTC ATg CAC TTg C | 2718 | |
| Q11S | ggT TTT CAg TgT gTT gAg ATg CTC AgC | 2908 | |
| Q10AS | gCA gCA ACC CCA AAg TAA TCg ATC | 2955 | |
| PCR6AS | CAg TgT gAT TTT TAA ggA CTg TC | 3271 | |
| hBUB1b | |||
| PCR-product 1 | |||
| PCR1S | TgT AgC TCC gAg ggC Agg | -97 | |
| Q1AS | CCT ATC CCA AAC ATC CAg Agg | 219 | |
| Q2S | gCT ggA CAg AgC AgA ACT ATC | 247 | |
| Q2AS | gCA TCT gCT TTC CTA AAg TTT TC | 495 | |
| PCR2AS | TTg AgA gCA CCT CCT ACA Cg | 729 | |
| PCR-product 2 | |||
| PCR3S | gAg TCT TCT gTA CCA CAA Cg | 651 | |
| Q4AS | gAA gCT gTA TTg CCA CgA gg | 966 | |
| Q4S | gTg gAA gAg ACT gCA CAA CAg C | 1029 | |
| Q5AS | CAC Tgg TCA ATA gCT Cgg CTT C | 1284 | |
| Q5S | CAg CAA gAA gAg ACg ATg CCT AC | 1398 | |
| Q6AS | ggA gAA Agg TAC ACT ggg ACC | 1566 | |
| PCR4AS | gAT ACA AAA CgA gCT gCT CTg gC | 1833 | |
| PCR-product 3a | |||
| PCR5S | TCA CAg gCT TCA gAA ATg TAA C | 1777 | |
| Q8AS | gTT TCT CAg gAA TTT gAA gAC | 2098 | |
| Q7S | gAA CTT ACT AAT gAg ACT TCA g | 2121 | |
| Q9AS | gCC AAA CAA TAC AgC CAT CTT g | 2484 | |
| Q10AS | gAA gAA CAg TTA gCC Agg ATC | 2819 | |
| PCR-product 3b | |||
| Q8S | CAA gAT ggC TgT ATT gTT Tgg C | 2484 | |
| Q9S | gTA gAC CTg TTT ggT ATA gCA g | 2850 | |
| Q11AS | ACA gCA CAT ACA gTT TCA gTg TTC | 3206 | |
| Q10S | gCT gCC TCA gAg CAA Tgg TTg | 3178 | |
| PCR6AS | ATA CAT ggT gAT CAT AAg AgA AC | 3464 | |
| hBUB3 | |||
| PCR1S | gTA gTg gAA ACg TTg CTT CTg Agg | -34 | |
| Q2S | CTg TCC AgA AgT gAA TgT gAT g | 311 | |
| Q1AS | CAA CTT CCA gTg ACC ATC AC | 327 | |
| Q3S | gAA gTA TgC CTT CAA ATg TCA C | 650 | |
| Q2AS | gCA TTg ACT ggg TAA ATC TgC TC | 693 | |
| PCR2AS | gAA ATC TTg TCA AgT ACA Tgg Tg | 973 | |
| TTK | |||
| PCR-product 1 | |||
| PCR1S | Tgg AAT CCg Agg ATT TAA gTg gC | -47 | |
| Q10AS | gTA gTC ACg TgC ATC ATC Tgg | 324 | |
| Q1S | gCT ATT CAA gAg CCA gAT gAT gC | 312 | |
| Q9AS | CTg TAA ATg CCC AAg TgA ACC | 600 | |
| Q2S | CCA CAA gAT gCA gAA ATA gg | 693 | |
| Q8AS | gAA TCA gTA ATA ATA AgT TCA g | 988 | |
| Q3S | gAg TAT CAA gAA CCA gAg gTT C | 1068 | |
| PCR1AS | gAA AgT CAT TCT TTA CAA CTg | 1357 | |
| PCR-product 2 | |||
| PCR2S | CCT Tgg ATg ATT ACA TgA gCT g | 1324 | |
| QextraS | gAA TgC ATT TCg gTT AAA ggA Ag | 1497 | |
| Q4S | ggA CCA gTA CAT CTA CAT gg | 1733 | |
| Q7AS | CCA TTA CCA TgT AgA TgT ACT g | 1737 | |
| Q5S | CCA AAA gTg ATg TTT ggT CC | 2089 | |
| Q6AS | CAT gAT TAg gAT CAA TTA Tgg C | 2187 | |
| PCR2AS | ggA TTC AAg AgT ATA ACA gTC C | 2570 | |
| Microsatellite primers (LOH-analysis) | |||
| Gene | GenMap'99 notation | Sequence | |
| hBUB1 | D2-S293 S | 5’6-FAM-AgC AAg TgC CTA TTT gAC ATC | |
| D2-S293 AS | CCA gAg TAT CgT gAg TgT gTg | ||
| hBUB1b | D15-S146 S | 5’HEX-ggA AgC CTg ACT TTT ATA TCC g | |
| D15-S146 AS | ATg TCT gTT CAg ATC CTT TgC | ||
| hBUB3 | D10-S587 S | 5’6-FAM-CCC AgA TTC ATg gCT TTC | |
| D10-S587 AS | TTC TgC TgA CAC ggg C | ||
| TTK | D6-S284 S | 5’6-FAM-CAT ggC TgT CTA TCA AAC CC | |
| D6-S284 AS | AAg CAT TTg TgT ggC TCT Tg | ||
Updated:
26 April, 2010
MOMA,
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