Aarhus University Hospital and Aarhus University
MOMA Core Center for "Next Generation Sequencing"

What is NGS?

"Next generation" or "deep" sequencing (NGS) refers to a new, versatile technology of DNA sequencing that permits high-throughput, highly adaptable whole genome-scale assays at reasonable cost and high accuracy.

NGS revolutionizes genomic research by setting a new standard. NGS has a tremendous potential for new discovery in multiple biological science disciplines.

This technology enables massively parallel sequencing of nucleic acid fragments to address a variety of experimental questions.

The ultra-high-throughput and accuracy of NGS opens countless possibilities of genome-scale assays in clinical, medical, cancer and basic research, screening large numbers of samples for changes underpinning diseases and traits of interest.

New systems and platforms, fast evolving methodologies, bioinformatics and innovative application of NGS in combination with other technologies change the landscape of what is possible today.

MOMA NGS Core Center
Aarhus University Hospital, The Faculty of Health at Aarhus University, and scientists at MOMA established the MOMA core center for NGS.

The NGS core center was opened on January, 12th, 2012 with representants from Aarhus University Hospital and Aarhus University and many interested guests.

The technology is moving fast ahead: new protocols and new equipment are introduced monthly.

MOMA has decided to focus on the market leader technologies and offer these as a core center service.

The amount of generated data is much larger than usual - therefore MOMA performs the first level of bioinformatics data analysis by highly dedicated staff. The subsequent downstream analyses are done by the user of the center.

Technology  
The NGS core center at MOMA provides massive parallel sequencing using a HiSeq2000, a Genome Analyzer IIx or a MiSeq. All instruments are supplied and supported by Illumina, Inc. and are fully updated with most recent software and hardware. Please see www.illumina.com for additional information on performance and specifications.

Services
We provide a range of standard Next Generation Sequencing (NGS) services.
At present we offer:

• Genome sequencing
• Exome sequencing
• RNA sequencing
• Initial data processing

Procedure
Please contact us for more information about our services and prices.

We will arrange a short meeting to discuss your project in detail. This will cover the approach, targeting, library preparation and data handling. Our scientists will assist you in identifying the design that fits your project best.
A customer contract including a time schedule is required before your samples can be processed.

After completion of the deep sequencing analyses, we will perform the initial data processing. The data will be handed over to you personally as agreed upon.

Typical Workflow

• Contact us by mail or phone
• Meeting for protocol planning
• Sample submission & QC
• Primary data analysis
• Data handover
• Downstream data analysis by user

Please, see the document Sample Requirements and Data Analysis for further details.

For more information, please contact: Mette Gaustadnes email phone +45 7845 5305

Staff
Mette Gaustadnes, PhD
Søren Vang, PhD, data manager
Kasper Thorsen, PhD
Jakob Hedegaard, PhD
Friedrik Wikman, Lic.scient.
Jakob Skou Pedersen, PhD, assoc. prof. in bioinformatics
Lone Andersen, med. technologist
Hanne Steen, med. technologist