Molecular Diagnostic Laboratory
Department of Molecular Medicine,
Aarhus University Hospital Skejby

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Free NormFinder software
NormFinder is an algorithm for identifying the optimal normalization gene among a set of candidates.
It ranks the set of candidate normalization genes according to their expression stability in a given sample set and given experimental design.

NormFinder can analyze expression data obtained through any quantitative method e.g. real time RT-PCR and microarray based expression analysis.

"NormFinder.xla" adds the NormFinder functionality directly to Excel.
The software is free for academic and commercial use.

Find more information in the documentation, the FAQ and the original article below.

Download MS Excel Add-in

2005-jan-05    Version 0.953
2004-sep-14   Version 0.952
2004-aug-19   Version 0.93

Documentation
Normfinder HowTo v20
updated May 2010

FAQ
updated January 2012

Download version for R

updated 2005-02-16

Example data (Excel format)

updated June 2009

This dataset illustrates, how to plot your data, estimate variances and evaluate normalization genes in your dataset.






 

 

 

 

 

 

Andersen C.L., Ledet-Jensen J., Ørntoft T.: Normalization of real-time quantitative RT-PCR data: a model based variance estimation approach to identify genes suited for normalization - applied to bladder- and colon-cancer data-sets.
Cancer Research. 2004 (64): 5245-5250 PubMed Supplementary data

Send your questions, comments or feedback to normfinder@mdl.dk.

Several alternative normalization software is available, find more information here.

 


Updated:28 December, 2011

 
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